Researchers found that male mice given kombucha showed fewer signs of depression-like behavior, no matter what type of diet they were fed. The study compared two groups of mice with different diets and tracked their responses over time.
Study design and dietary groups
The experiment divided mice into two groups based on diet: one group ate a standard lab feed, while the other followed a Western-style diet high in fat and sugar. Within each group, half received kombucha daily for seven weeks, and the other half drank only water.
After the feeding period, scientists conducted behavioral tests to assess anxiety and depression-like responses. They also collected intestinal samples to examine microbial changes and analyzed biochemical markers in both gut and brain tissues.
Kombucha consumption led to better performance in depression-related tests for all mice, regardless of their diet. The improvement in depression-like behavior was consistent across both dietary groups.
Only the mice on the Western diet that drank kombucha showed notable improvements in anxiety-related measures. Those on the standard diet did not experience significant changes in anxiety-like responses.
Microbial analysis indicated that kombucha drinkers had higher levels of bacteria associated with tryptophan metabolism. Tryptophan serves as a precursor to serotonin, a key neurotransmitter in mood regulation.
The diet itself influenced other biochemical outcomes. Mice on the standard diet had higher levels of short-chain fatty acids, which help maintain intestinal barrier function. Meanwhile, those on the Western diet showed increased brain levels of tryptophan and its metabolite kynurenine, along with a smaller spleen size, suggesting changes in immune function.
The researchers noted that mouse behavior does not necessarily reflect human emotional experiences. While the tests identify similar patterns, they cannot confirm whether kombucha improves mood in people.
According to the study authors, human clinical trials are needed to determine if the beverage provides measurable mental health benefits. Until then, kombucha should be considered a flavorful part of a varied diet rather than a treatment for anxiety or depression.
Individuals struggling with persistent low mood or anxiety should seek professional mental health care and follow prescribed therapies, as kombucha cannot replace established medical approaches.